RESEARCH ONLY Research-grade reference compounds, supplied for laboratory research use only. Not for human consumption.
ATTENTION ALL RESEARCHERS

Get Your Ratios 100% Correct

To reconstitute a freeze-dried powder or peptide, the basic ratio is Total Mass (mg) divided by Volume of Water (mL). On a standard U-100 insulin syringe, 1 mL equals 100 units.

Core Reconstitution Formulas

1. Solution Concentration

Concentration (mg/mL) = Total Mass (mg) ÷ Water Volume (mL)

2. Microgram (mcg) Conversion

Mass in mcg = Mass in mg × 1,000
e.g. 1 mg = 1,000 mcg

3. Syringe Units to Draw (U-100 Syringe)

Syringe Units = [ Target Dose (mg) ÷ Concentration (mg/mL) ] × 100

Common Easy-to-Calculate Ratios for Popular Vials

Bookmark these standardized ratios for rapid calculation during research protocols:

5 mg Vial + 1 mL Water

Conc: 5 mg/mL
  • 10 Units on Syringe = 0.5 mg (500 mcg)
  • 20 Units on Syringe = 1.0 mg (1,000 mcg)

5 mg Vial + 2 mL Water

Conc: 2.5 mg/mL
  • 10 Units on Syringe = 0.25 mg (250 mcg)
  • 20 Units on Syringe = 0.5 mg (500 mcg)

10 mg Vial + 2 mL Water

Conc: 5 mg/mL
  • 20 Units on Syringe = 1.0 mg (1,000 mcg)
  • 40 Units on Syringe = 2.0 mg (2,000 mcg)

10 mg Vial + 1 mL Water

Conc: 10 mg/mL
  • 10 Units on Syringe = 1.0 mg (1,000 mcg)
  • 20 Units on Syringe = 2.0 mg (2,000 mcg)

4 Basic Mixing Steps for Laboratory Preparation

1

Wipe Clean

Clean the rubber stoppers of both your reference vial and the Bacteriostatic Water vial thoroughly with an alcohol swab.

2

Equalize Pressure

Draw air into your syringe equal to the water volume to add, inject it into the water vial, and draw up the target water volume.

3

Add Gently

Insert needle at an angle. Let the water stream slide down the inside glass wall slowly instead of blasting the lyophilized powder directly.

4

Mix Thoroughly

Swirl the vial gently until the liquid solution is completely clear. Never shake the vial to protect protein structures.

Need a Custom Vial Size or Water Volume Calculation?

Enter your specific vial mg and BAC water mL into our live interactive tool for instant syringe unit outputs.

Launch Interactive Reconstitution Calculator

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